ANTICODON STEM-LOOP TRNA MODIFICATIONS INFLUENCE CODON DECODING AND FRAME MAINTENANCE DURING TRANSLATION

2022

TJ Smith and KS Koutmou

submitted

N1-METHYLPSEUDOURIDINE AND PSEUDOURIDINE MODIFICATIONS IMPACT AMINO ACID MISINCORPORATION DURING TRANSLATION

2022

JG Monroe, L Mitchell, I Deb, B Roy, AZ Frank, KS Koutmou

submitted, under revision (pre-print on bioRxiv DOI: 10.1101/2022.06.13.495988)

SEQUENCE-SPECIFIC PROGRAMMABLE CAPTURE AND CONCENTRATION OF VIRAL RNA BY TYPE III CRISPR SYSTEM ENHANCES DIAGNOSTIC

2022

A Nemudraia, A Nemudryi, M Buyukyoruk, AM Scherffius, T Zahl, T Wiegand, S Pandey, J Nichols, L Hall, A McVey, HH Lee, R Wilkinson, LR Snyder, JD Jones, KS Koutmou, A Santiago-Frangos, B Wiedenheft

submitted, under revision (pre-print on bioRxiv DOI: 10.21203/rs.3.rs-1466718/v1)

METHYLATED GUANOSINE AND URIDINE MODIFICATIONS IN S. CEREVISIAE MRNAS MODULATE TRANSLATION

2022

JD Jones, MK Franco, TJ Smith, LR Snyder, RT Kennedy, KS Koutmou

submitted, under revision (pre-print on bioRxiv DOI: 10.1101/2022.06.13.495843)

A HUMANIZED YEAST MODEL REVEALS DOMINANT-NEGATIVE PROPERTIES OF NEUROPATHY-ASSOCIATED ALANYL-TRNA SYNTHETASE MUTATIONS

2022

R Meyer-Schuman, S Marte, TJ Smith, S Feely, M Kennerson, G Nicholson, M Shy, KS Koutmou, A Antonellis.

submitted, under revision (pre-print on bioRxiv DOI: 10.1101/2022.05.25.493316)

MAPPING MRNA MODIFICATIONS FOR FUNCTIONAL STUDIES

2022

JD Jones, DE Eyler, KS Koutmou

Nature Biotechnology, in press

SUPPORTING STUDENT LEARNING IN BIOCHEMISTRY USING A SCIENCE COMMUNICATION FOCUSED WRITING ASSIGNMENT

2022

SA Finkenstaedt-Quinn, NF Garza, CA Wilhelm, KS Koutmou, GV Shultz

Journal of Chemical Education (DOI: 10.1021/acs.jchemed.2c00171)

CGG REPEATS TRIGGER TRANSLATIONAL FRAMESHIFTING TO GENERATE AGGREGATION-PRONE CHIMERIC PROTEINS IN NEURONS

2022

SE Wright, CM Rodriguez, J Monroe, J Xing, A Krans, BN Flores, V Barsur, MI Ivanova, KS Koutmou, SJ Barmada, PK Todd

Nucleic Acids Research (DOI: 10.1093/nar/gkac626)

MRNA AND TRNA MODIFICATION STATES INFLUENCE RIBOSOME SPEED AND FRAME MAINTENANCE DURING POLY(LYSINE) PEPTIDE SYNTHESIS

2022

TJ Smith, M Tardu, HJ Khatri, KS Koutmou

Journal of Biological Chemistry (DOI: 10.1016/j.jbc.2022.102039)

***Selected as an Editor's Choice article

CHEMICAL MODIFICATIONS TO MRNA NUCLEOBASES IMPACT TRANSLATION ELONGATION AND TERMINATION

2022

MK Franco and KS Koutmou

Biophysical Chemistry (DOI: 10.1016/j.bpc.2022.106780)

***Article featured on the journal cover

THE CAMP SIGNALING PATHWAY REGULATES EPE1 PROTEIN LEVELS AND HETEROCHROMATIN ASSEMBLY

2022

S Jia, K Bao, CM Shan, X Chen, G Raiymbek, JG Monroe, KS Koutmou, K Ragunathan, C Lu, L Berchowitz

PLoS Genetics (DOI: 10.1371/journal.pgen.1010049)

PSEUDOURIDINE SYNTHASE 7 IS AN OPPORTUNISTIC ENZYME THAT BINDS AND MODIFIES SUBSTRATES WITH DIVERSE SEQUENCES AND STRUCTURES

2022

MK Purchal, DE Eyler, M Tardu, MK Franco, MM Korn, T Khan, R McNassor, R Giles, K Lev, H Sharma, J Monroe, L Mallik, M Koutmos, KS Koutmou

PNAS (DOI: 10.1073/pnas.2109708119)

IN VITRO INVESTIGATION OF TRANSLATIONAL FIDELITY ON MODIFIED CODONS

2021

JG Monroe, TJ Smith, KS Koutmou

Methods Enzymology (DOI: 10.1016/bs.mie.2021.06.011)

COMMUNICATING SCIENCE TO THE GENERAL PUBLIC THROUGH A BIOCHEMISTRY WRITING ASSIGNMENT

2021

Garza N, Finkenstaedt-Quinn S, Wilhelm, C, Koutmou K, Shultz G

Journal of Chemical Education (DOI: 10.1021/acs.jchemed.0c01176)

A MOLECULAR LEVEL PERSPECTIVE ON THE FREQUENCY, DISTRIBUTION AND POSSIBLE CELLULAR ROLES OF MRNA MODIFICATIONS

2020

JD Jones, J Monroe and KS Koutmou

WIREs RNA (DOI: 10.1002/wrna.1586)

***Recognized as a “top cited article 2020-2021” in WIREs RNA

PSEUDOURIDINYLATION OF MRNA CODING SEQUENCES ALTERS TRANSLATION

2019

DE Eyler, MK Franco, Z Batool, MZ Wu, ML Debuke, M Dobosz-Bartoszek, JD Jones, Y Polikanov, B Roy, KS Koutmou

PNAS (DOI: 10.1073/pnas.1821754116)

IDENTIFICATION AND QUANTIFICATION OF MODIFIED NUCLEOSIDES IN SACCHAROMYCES CEREVISIAE MRNAS

2019

M Tardu, JD Jones, RT Kennedy, Q Lin, KS Koutmou

ACS Chemical Biology (DOI: 10.1021/acschembio.9b00369)

SYNTHESIS AT THE SPEED OF CODONS

2015

KS Koutmou, A Radhakrishnan, R Green

Trends in Biomedical Sciences

TRANSLATIONAL CONTROL BY LYSINE-ENCODING A-RICH SEQUENCES

2015

L Arthur, S Pavlovic-Djuranovic, KS Koutmou, R Green, P Szczesny, S Djuranovic

Science Advances

SID-1 EXTRACELLULAR DOMAIN SELECTIVELY BINDS LONG DSRNA AND IS REQUIRED FOR RNA TRANSPORT BY SID-1

2015

W Li, KS Koutmou, DJ Leahy, M Li

Journal of Biological Chemistry

RIBOSOMES SLIDE ON LYSINE-ENCODING HOMOPOLYMERIC A STRETCHES

2015

KS Koutmou, AP Schuller, JL Brunelle, A Radhakrishnan, S Djuranovic, R Green

eLife

RF3:GTP PROMOTES RAPID DISSOCIATION OF THE CLASS 1 TERMINATION FACTOR

2014

KS Koutmou, ME McDonald, JL Brunelle, R Green

RNA

THE RNR MOTIF OF B. SUBTILIS RNASE P PROTEIN INTERACTS WITH BOTH PRNA AND PRE-TRNA TO STABILIZE AN ACTIVE CONFORMER

2011

KS Koutmou, JJ Day-Storms, CA Fierke

RNA

A DIVALENT CATION STABILIZES THE ACTIVE CONFORMATION OF THE B. SUBTILIS RNASE P•PRE-TRNA COMPLEX: A ROLE FOR AN INNER-SPHERE METAL ION IN RNASE P

2010

J Hsieh, KS Koutmou, D Rueda, M Koutmos, NG Walter, CA Fierke

Journal of Molecular Biology

NMR AND XAS REVEAL AN INNER-SPHERE METAL BINDING SITE IN THE P4 HELIX OF THE METALLO-RIBOZYME RIBONUCLEASE P

2010

KS Koutmou, A Casiano-Negroni, M Getz, S Paczini, AJ Andrews, JE Penner-Hahn, HM Al-Hashimi, CA Fierke

Proceedings of the National Academy of Sciences

PROTEIN–PRECURSOR TRNA CONTACT LEADS TO SEQUENCE-SPECIFIC RECOGNITION OF 5′ LEADERS BY BACTERIAL RIBONUCLEASE P

2010

KS Koutmou, NH Zahler, JC Kurz, FE Campbell, ME Harris, CA Fierke

Journal of Molecular Biology

KINETIC MECHANISM OF BACTERIAL RNASE P

2009

KS Koutmou, J Hsieh, CA Fierke

Protein Reviews, Volume 10: Ribonuclease P (book chapter)

IMPORTANCE OF RNA-PROTEIN INTERACTIONS IN BACTERIAL RIBONUCLEASE P STRUCTURE AND CATALYSIS

2007

JK Smith, J Hsieh, CA Fierke

Biopolymers