Chemical modifications to mRNA nucleobases impact translation elongation and termination


MK Franco and KS Koutmou

Biophysical Chemistry, in press

The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly


S Jia, K Bao, CM Shan, X Chen, G Raiymbek, JG Monroe, KS Koutmou, K Ragunathan, C Lu, L Berchowitz

PLoS Genetics, in press

Pseudouridine synthase 7 is an opportunistic enzyme that binds and modifies substrates with diverse sequences and structures


MK Purchal, DE Eyler, M Tardu, MK Franco, MM Korn, T Khan, R McNassor, R Giles, K Lev, H Sharma,  J Monroe, L Mallik, M Koutmos, KS Koutmou

PNAS (DOI: 10.1073/pnas.2109708119)

Sequence and modification status of RNAs in the translation machinery direct ribosome movements during the synthesis of poly(lysine) encoding peptides


TJ Smith, M Tardu, KS Koutmou

under revision

Methylated guanosine and uridine modifications in S. cerevisiae mRNAs modulate translation


JD Jones, MK Franco, TJ Smith, LR Snyder, RT Kennedy, KS Koutmou

under revision

Supporting student learning in biochemistry using a science communication focused writing assignment


SA Finkenstaedt-Quinn, NF Garza, CA Wilhelm, KS Koutmou, GV Shultz


In vitro investigation of translational fidelity on modified codons


JG Monroe, TJ Smith, KS Koutmou

Methods Enzymology (DOI: 10.1016/bs.mie.2021.06.011)

Communicating science to the general public through a biochemistry writing assignment


Garza N, Finkenstaedt-Quinn S, Wilhelm, C, Koutmou K, Shultz G

Journal of Chemical Education (DOI: 10.1021/acs.jchemed.0c01176)

A molecular level perspective on the frequency, distribution and possible cellular roles of mRNA modifications


JD Jones, J Monroe and KS Koutmou

WIREs RNA (DOI: 10.1002/wrna.1586)

Pseudouridinylation of mRNA coding sequences alters translation


DE Eyler, MK Franco, Z Batool, MZ Wu, ML Debuke, M Dobosz-Bartoszek, JD Jones, Y Polikanov, B Roy, KS Koutmou

PNAS (DOI: 10.1073/pnas.1821754116)

Identification and quantification of modified nucleosides in Saccharomyces cerevisiae mRNAs


M Tardu, JD Jones, RT Kennedy, Q Lin, KS Koutmou

ACS Chemical Biology (DOI: 10.1021/acschembio.9b00369)

Synthesis at the speed of codons


KS Koutmou, A Radhakrishnan, R Green

Trends in Biomedical Sciences

Translational control by lysine-encoding A-rich sequences


L Arthur, S Pavlovic-Djuranovic, KS Koutmou, R Green, P Szczesny, S Djuranovic

Science Advances

SID-1 extracellular domain selectively binds long dsRNA and is required for RNA transport by SID-1


W Li, KS Koutmou, DJ Leahy, M Li

Journal of Biological Chemistry

Ribosomes slide on lysine-encoding homopolymeric A stretches


KS Koutmou, AP Schuller, JL Brunelle, A Radhakrishnan, S Djuranovic, R Green


RF3:GTP promotes rapid dissociation of the class 1 termination factor


KS Koutmou, ME McDonald, JL Brunelle, R Green


The RNR motif of B. subtilis RNase P protein interacts with both PRNA and pre-tRNA to stabilize an active conformer


KS Koutmou, JJ Day-Storms, CA Fierke


A divalent cation stabilizes the active conformation of the B. subtilis RNase P•pre-tRNA complex: a role for an inner-sphere metal ion in RNase P


J Hsieh, KS Koutmou, D Rueda, M Koutmos, NG Walter, CA Fierke

Journal of Molecular Biology

NMR and XAS reveal an inner-sphere metal binding site in the P4 helix of the metallo-ribozyme ribonuclease P


KS Koutmou, A Casiano-Negroni, M Getz, S Paczini, AJ Andrews, JE Penner-Hahn, HM Al-Hashimi, CA Fierke

Proceedings of the National Academy of Sciences

Protein–precursor tRNA contact leads to sequence-specific recognition of 5′ leaders by bacterial ribonuclease P


KS Koutmou, NH Zahler, JC Kurz, FE Campbell, ME Harris, CA Fierke

Journal of Molecular Biology

Kinetic mechanism of bacterial RNase P


KS Koutmou, J Hsieh, CA Fierke

Protein Reviews, Volume 10: Ribonuclease P (book chapter)

Importance of RNA-protein interactions in bacterial ribonuclease P structure and catalysis


JK Smith, J Hsieh, CA Fierke